The humanized antibody essentially refers to that which is re-explained by the monoclonal immunoglobulin by gene twinning and DNA recombination process, and most of the amino acid sequence is substituted by the human sequence, and the affinity and specificity of the parent mouse monoclonal are substantially retained, and the antibody is reduced. Its heterogeneity is beneficial to the human body.
The variable region of the angiotensin receptor antibody gene is recombined with the constant region of the human antibody gene by DNA recombination technology, and the recombinant gene is introduced into the myeloma cell for expression.
Depending on the vector plasmid used to label the gene product, an appropriate antibiotic or preparation is used for screening, and a cell line secreting the human-mouse chimeric one is cloned in a similar manner to the conventional technique.
2. CDR-grafted humanized antibodies
The FR in the V region of the murine antibody still retains some immunogenicity. This is far from being a truly humanized antibody, and some can produce a strong anti-idiotype response.
In order to reduce the composition of the mouse, people try to replace the FR of the mouse with human FR to form a more complete antibody humanization service antibody, that is to say, except that the three CDRs are murine sources, all of them are human structures, also known as CDR-grafted antibodies. Or modified antibodies
3. Fully humanized antibody
The production of phage antibody library technology relies on the development of three experimental techniques: one is the development of PCR technology that allows one to clone a complete set of immunoglobulin variable regions from B lymphocyte total RNA by RP-TCR. Genes, making the construction of antibody libraries simple and easy to manipulate.